Publications

2022

Molinar Jr., T., Sultanov, D., Klein, H., Hochwagen, A. Topoisomerase I promotes formation of a novel DNA intermediate in the ribosomal DNA. bioRxiv 

Sultanov, D., & Hochwagen, A. Deep selection shapes the intragenomic diversity of rRNA genes. bioRxiv 

Kar, F. M., Vogel, C., & Hochwagen, A. (2022). Meiotic DNA breaks activate a streamlined phospho-signaling response that largely avoids protein level changes. bioRxiv

Heldrich, J., Milano, C. R., Markowitz, T. E., Ur, S. N., Vale-Silva, L. A., Corbett, K. D., & Hochwagen, A. (2022). Two pathways drive meiotic chromosome axis assembly in Saccharomyces cerevisiae. Nucleic acids research50(8), 4545–4556. Pubmed

2021

Kar, FM, and Hochwagen A. 2021. Phospho-Regulation of Meiotic Prophase. Frontiers in Cell and Developmental Biology 9 (April): 667073. PubMed

2020

Heldrich J, Markowitz TE, Vale-Silva LA, Hochwagen A. (2020) A cohesin-independent mechanism modulates recombination activity along meiotic chromosomes. bioRxiv:2020.08.11.247122. bioRxiv

Markowitz TE, Heldrich J, Hochwagen A. (2020) DNA break formation induces Scc2/cohesin-dependent recruitment of condensin to meiotic chromosomes. bioRxiv:2020.07.16.207068. bioRxiv

González-Arranz S, Gardner JM, Yu Z, Patel NJ, Heldrich J, Santos B, Carballo JA, Jaspersen SL, Hochwagen A, & San-Segundo PA (2020). SWR1-Independent Association of H2A.Z to the LINC Complex Promotes Meiotic Chromosome Motion. Frontiers in Cell and Developmental Biology, 8, 594092.bioRxiv PubMed

Heldrich J, Sun X, Vale-Silva LA, Markowitz TE, Hochwagen A. (2019) Topoisomerases modulate the timing of meiotic DNA breakage and chromosome morphogenesis in Saccharomyces cerevisiae. bioRxiv:672337. bioRxiv Genetics 10.1534. Full Text

2019

De Ioannes P LV, Leon VA, Kuang Z, Wang M, Boeke JD, Hochwagen A, Armache KJ. (2019) Structure and function of the Orc1 BAH-nucleosome complex. Nat Commun 10:2894. Pubmed

Subramanian VV, Zhu X., Markowitz TE, Vale-Silva LA, San-Segundo PA, Hollingsworth NM, Keeney S, Hochwagen A. (2019) Persistent DNA-break potential near telomeres increases initiation of meiotic recombination on short chromosomes. Nat Commun 10: 970. PubMed PDF bioRxiv:201889. bioRxiv

Paul MR, Hochwagen A, Ercan S. (2019). Condensin action and compaction. Curr Genet (2019) 65: 407. PDF

Vale-Silva LA, Markowitz TE, Hochwagen A. (2019) SNP-ChIP: a versatile and tag-free method to quantify changes in protein binding across the genome. BMC Genom. 20, 54. PubMed PDF

2018

Mansisidor AR, Molinar Jr. T, Srivastava P, Blitzblau H, Klein H, Hochwagen A. (2018) Genomic copy-number loss is rescued by self-limiting production of DNA circles.  Mol Cell. 2018 Nov 1;72(3):583-593.e4.  bioRxiv:255471. bioRxiv PDF

2017

Paul MR, Markowitz TE, Hochwagen A, Ercan S. (2017) Acute condensin depletion causes genome decompaction without altering the level of global gene expression in Saccharomyces cerevisiae. bioRxiv:195487. bioRxiv

Markowitz TE, Suarez D, Blitzblau HG, Patel NJ, Markhard AL, MacQueen AJ, Hochwagen A. (2017) Reduced dosage of the chromosome axis factor Red1 selectively disrupts the meiotic recombination checkpoint in Saccharomyces cerevisiae. PLoS Genet, 13(7):e1006928 PubMed PDF

Argunhan B, Leung WK, Afshar N, Terentyev Y, Subramanian VV, Murayama Y, Hochwagen A, Iwasaki H, Tsubouchi T, Tsubouchi H. (2017) Fundamental cell cycle kinases collaborate to ensure timely destruction of the synaptonemal complex during meiosis. EMBO J, 36(17):2488-2509 PubMed

2016

Subramanian VV, MacQueen AJ, Vader G, Shinohara M, Sanchez A, Borde V, Shinohara A, Hochwagen A. (2016) Chromosome Synapsis Alleviates Mek1-Dependent Suppression of Meiotic DNA Repair. PLoS Biol, 14(2):e1002369 PubMed PDF

Wang D, Mansisidor A, Prabhakar G, Hochwagen A. (2016) Condensin and Hmo1 Mediate a Starvation-Induced Transcriptional Position Effect within the Ribosomal DNA Array. Cell Rep, 14:1010-1017 PubMed PDF

He H, Zhang S, Wang D, Hochwagen A, Li F. (2016) Condensin Promotes Position Effects within Tandem DNA Repeats via the RITS Complex. Cell Rep, 14:1018-1024 PubMed PDF

2015

Vincenten N, Kuhl LM, Lam I, Oke A, Kerr AR, Hochwagen A, Fung J, Keeney S, Vader G, Marston AL. (2015) The kinetochore prevents centromere-proximal crossover recombination during meiosis. eLife 2015;4:e10850 PubMed PDF

Gothwal SK, Patel NJ, Colletti MM, Sasanuma H, Shinohara M, Hochwagen A, Shinohara A. (2015) The Double-Strand Break Landscape of Meiotic Chromosomes Is Shaped by the Paf1 Transcription Elongation Complex in Saccharomyces cerevisiae. Genetics, 202(2):497-512PubMed

Sun X, Huang L, Markowitz TE, Blitzblau HG, Chen D, Klein F, Hochwagen A. (2015) Transcription dynamically patterns the meiotic chromosome-axis interface. eLife 2015;4:e07424 PubMed PDF

Voelkel-Meiman K, Johnston C, Thappeta Y, Subramanian VV, Hochwagen A, MacQueen AJ. (2015) Separable Crossover-Promoting and Crossover-Constraining Aspects of Zip1 Activity during Budding Yeast Meiosis. PLoS Genet, 11(6):e1005335 PubMed PDF

2014

Humphryes N, Hochwagen A. (2014) A non-sister act: Recombination template choice during meiosis. Exp Cell Res, 15;329(1):53-60PubMed

Subramanian VV, Hochwagen A. (2014) The Meiotic Checkpoint Network: Step-by-Step through Meiotic Prophase. Cold Spring Harb Perspect Biol 6(10):a016675 PubMed

2013

Copsey A, Tang S, Jordan PW, Blitzblau HG, Newcombe S, et al. (2013) Smc5/6 Coordinates Formation and Resolution of Joint Molecules with Chromosome Morphology to Ensure Meiotic Divisions. PLoS Genet 9(12): e1004071 PubMed PDF

Blitzblau HG, Hochwagen A (2013) ATR/Mec1 prevents lethal meiotic recombination initiation on partially replicated chromosomes in budding yeast. eLife 2013;2:e00844 PubMed PDF

2012

Agarwala SD, Blitzblau HG, Hochwagen A, Fink GR (2012) RNA Methylation by the MIS Complex Regulates a Cell Fate Decision in Yeast.PLoS Genet 8(6): e1002732. PubMed PDF

Blitzblau HG, Chan CS, Hochwagen A, Bell SP (2012) Separation of DNA Replication from the Assembly of Break- Component Meiotic Chromosomes. PLoS Genet 8(5): e1002643. PubMed PDF

2011

Subramanian, V.V., and Hochwagen, A. (2011). Centromere clustering: where synapsis begins. Curr Biol 21, R920-922. PubMed

Vader, G., Blitzblau, H.G., Tame, M.A., Falk, J.E., Curtin, L., and Hochwagen, A. (2011). Protection of repetitive DNA borders from self-induced meiotic instability. Nature 477, 115-119. PubMed PDF Supplemental Data PDF

Blitzblau, H.G., and Hochwagen, A. (2011). Genome-wide detection of meiotic DNA double-strand break hotspots using single-stranded DNA. Methods Mol Biol 745, 47-63. PubMed PDF

MacQueen, A.J., and Hochwagen, A. (2011). Checkpoint mechanisms: the puppet masters of meiotic prophase. Trends Cell Biol 21, 393-400.PubMed

Pan, J., Sasaki, M., Kniewel, R., Murakami, H., Blitzblau, H.G., Tischfield, S.E., Zhu, X., Neale, M.J., Jasin, M., Socci, N.D., Hochwagen, A., Keeney, S. (2011). A hierarchical combination of factors shapes the genome-wide topography of yeast meiotic recombination initiation.Cell 144, 719-731. PubMed PDF Supplemental Data PDF

2010

Falk, J.E., Chan, A.C., Hoffmann, E., and Hochwagen, A. (2010). A Mec1- and PP4-dependent checkpoint couples centromere pairing to meiotic recombination. Dev Cell 19, 599-611. PubMed

Hochwagen, A., and Marais, G.A. (2010). Meiosis: a PRDM9 guide to the hotspots of recombination. Curr Biol 20, R271-274. PubMed

2009

Hochwagen, A. (2009). Meiosis: making a synaptonemal complex just got easier. Curr Biol 19, R849-851. PubMed

Brar, G.A., Hochwagen, A., Ee, L.S., and Amon, A. (2009). The multiple roles of cohesin in meiotic chromosome morphogenesis and pairing.Mol Biol Cell 20, 1030-1047. PubMed PDF

2008

Chen, S.Y., Tsubouchi, T., Rockmill, B., Sandler, J.S., Richards, D.R., Vader, G., Hochwagen, A., Roeder, G.S., and Fung, J.C. (2008). Global analysis of the meiotic crossover landscape. Dev Cell 15, 401-415. PubMed PDF Supplemental Data PDF

Hochwagen, A. (2008). Meiosis. Curr Biol 18, R641-R645. PubMed

2007

Blitzblau, H.G., Bell, G.W., Rodriguez, J., Bell, S.P., and Hochwagen, A. (2007). Mapping of meiotic single-stranded DNA reveals double-stranded-break hotspots near centromeres and telomeres. Curr Biol 17, 2003-2012. PubMed

2006

Hochwagen, A., and Amon, A. (2006). Checking your breaks: surveillance mechanisms of meiotic recombination. Curr Biol 16, R217-228.PubMed

2005

Hochwagen, A., Tham, W.H., Brar, G.A., and Amon, A. (2005). The FK506 binding protein Fpr3 counteracts protein phosphatase 1 to maintain meiotic recombination checkpoint activity. Cell 122, 861-873. PubMed

Hochwagen, A., Wrobel, G., Cartron, M., Demougin, P., Niederhauser-Wiederkehr, C., Boselli, M.G., Primig, M., and Amon, A. (2005). Novel response to microtubule perturbation in meiosis. Mol Cell Biol 25, 4767-4781. PubMed PDF

2002

Haering, C.H., Lowe, J., Hochwagen, A., and Nasmyth, K. (2002). Molecular architecture of SMC proteins and the yeast cohesin complex. Mol Cell 9, 773-788. PubMed

2000

Panizza, S., Tanaka, T., Hochwagen, A., Eisenhaber, F., and Nasmyth, K. (2000). Pds5 cooperates with cohesin in maintaining sister chromatid cohesion. Curr Biol 10, 1557-1564. PubMed